Isolation and identification of <i>Bacillus subtilis</i> and its potential in biocontrol of phytopathogenic fungi
Abstract
In the work, based on morphological, cultural, biochemical properties and on the basis of the 16S rRNA gene, a local strain of bacteria Bacillus subtilis TM was isolated from saline soils of the Fergana region of Uzbekistan. The strain was registered with NCBI under the number: OR944512.1. It was revealed that the strain Bacillus subtilis TM exhibits high antagonistic activity against the phytopathogenic fungi Fusarium verticillioides , Fusarium solani , Aspergillus orchaceus , Alternaria alternata , Fusarium culmorium , Alternaria tenuissima with an inhibition zone of 40-60 mm. At the same time, the B. subtilis TM strain showed moderate antagonistic activity against Fusarium oxysporium , Penicillium chrysogenium (with an inhibition zone of 22-23 mm) and relatively less antagonistic activity against Cladosporium sp (with an inhibition zone of 12 mm).