Simple Identification of Transgenic<i>Arabidopsis</i>Plants Carrying a Single Copy of the Integrated Gene
Tomonori KiharaForestry Research Institute, Oji Paper, Kameyama, Mie 519-0212, JapanCheng‐Ri ZhaoLaboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu UniversityYuriko KobayashiLaboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu UniversityEiji TakitaGraduate School of Biological Sciences, Nara Institute of Science and TechnologyTetsu KawazuForestry Research Institute, Oji PaperHiroyuki KoyamaLaboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University
ABI
Аннотация
Transgenic Arabidopsis thaliana plants carrying a single copy of integrated DNA can be identified by single-step genomic polymerase chain reaction. The reaction employs two sets of primer pairs with the same melting temperature that amplify the amplicons derived from the integrated T-DNA together with those from an endogenous single-copy gene as a reference. When the band intensity ratio is one, this means that the transgenic plants are carrying a single copy of the integrated gene per haploid.
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