IRON CHELATION OF ROSMARINIC ACID
Аннотация
Objective To investigate the iron-chelation properties of rosmarinic acid. Methods Orthophenanthroline spectrophometry was used to observe the iron chelation properties with different concentrations of rosmarinic acid,and western blots used to evaluate the regulation of ferroportin 1(FP1) by rosmarinic acid in MES23.5 cells.MTT assay was used to observe the changes of cell viability with different concentrations of rosmarinic acid incubation in MES23.5 cell. Results Orthophenanthroline experiment indicated that the concentrations of 10-4-10-2 mol/L rosmarinic acid showed significant iron chelation properties(F=7.851,q=3.88-6.14,P0.05),and that of 10-5 mol/L did not(P0.05).MTT assay showed that 10-5 and10-4 mol/L rosmarinic acid did not have any toxic effects on MES23.5 cells(P0.05).The cell viability decreased when treated with 10-3 mol/L rosmarinic acid(F=4.948,q=4.04,P0.05).10-4 mol/L rosmarinic acid had no effects on FP1 expression in MES23.5 cells(F=0.333,P0.05). Conclusion Rosmarinic acid has an evident chelation of ferri ion,which is of potential prospect in the treatment of iron-metabolism-related conditions.