Optimization of Development of Pomegranate (Punica granatum L.) Varieties from Microclonal Propagation
Аннотация
Background and Objective: Besides, increasing cost-efficiency of MS and WPM nutrient media for the regeneration of explants and rhizogenesis of microshoots was planned by introducing changes in the composition of phytohormones.Studying the effect of a supramolecular complex of glycyrrhizinic and salicylic acids on the rhizogenesis of microshoots was aimed in terms of economic benefits.The research was aimed at optimizing in vitro microclonal propagation of some pomegranate varieties (Punica granatum L.).Materials and Methods: August, Kazake-anar, Achikh-dona and Tuyatish varieties of local pomegranate were selected in the study.Various concentrations of sterilants were used for the surface sterilization of explants.Explants were grown in MS and WPM media containing various concentrations of cytokinin, auxin and gibberellic acid.The supramolecular complex was used for microshoot rhizogenesis.Moreover, optimal conditions for acclimatization in microplants were developed.Results: The addition of gibberellic acid into MS and WPM nutrition media did not result in significant differences in the microshoot length.But the addition speeded up the formation of buds.Only earlier development of buds in some varieties were observed.The supramolecular complex used in this work at 0.15 and 0.17 mg LG 1 led to several-fold improvement in root number and length.No significant changes were observed between MS and WPM culture media.Economically utilizing the supramolecular complex was about 45 times cheaper than the mixture of IBA and IAA that was added to MS and WPM nutrition media.Conclusion: An enhanced cost-efficiency of the technology that enables obtaining pomegranate microshoot during the whole year.