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Gas Chromatography–Mass Spectrometry Analysis and Bioactive Potential of Selected Medicinal Plants

Waheed Qamar KhanDepartment of Botany Islamia College Peshawar Peshawar PakistanMuhammad Saleem KhanDepartment of Botany Islamia College Peshawar Peshawar PakistanGhulam SaddiqDepartment of Physics Islamia College Peshawar Peshawar PakistanMuhammad Zafar-ul-HyeDepartment of Plant Sciences Quaid‐i‐Azam University Islamabad Islamabad PakistanSalman MajeedDepartment of Botany University of Mianwali Mianwali PakistanMohamed Fawzy RamadanDepartment of Clinical Nutrition, Faculty of Applied Medical Sciences Umm Al‐Qura University Makkah Kingdom of Saudi ArabiaMuhammad AliDepartment of Microbiology Quaid‐i‐Azam University Islamabad Islamabad PakistanNasibakhon NaraliyevaDepartment of Ecology and Botany Andijan State University UzbekistanTrobjon MakhkamovDepartment of Botany and Genetics National University of Uzbekistan Tashkent UzbekistanZulunov IslomDepartment of Ecology and Botany Andijan State University UzbekistanAneta A. PtaszyńskaDepartment of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology Maria Curie‐Skłodowska University Lublin Poland
ABI

Аннотация

Plants offer unlimited source of bioactive compounds that have tremendous applications in pharmaceutical industry to find new sources of medicinal plants (MPs) were evaluated. Gas chromatography-mass spectrometry were used to identify the bioactive compounds of selected medicinal plants. Both extracts contained multiple compounds. The most abundant ones were 3,4-dimethyl-1,5-hexadiene-3,4-diol (7.93%), tau-cadinol (5.84%), and adenosine, N6-phenylacetic acid (5.84%) in one extract. In the other extract, the major compounds were 2-methylcumarone (16.92%), 7-methylbenzo[b]furan (14.81%), and (3E,5E)-3,5-octadien-2-one (10.25%). Some of these compounds have antimicrobial, anticancer, and antioxidant properties. Antioxidant tests showed that Solanum nigrum had the highest antioxidant activity (96.59% at 14.4 concentration), followed closely by Artemisia scoparia (96.55% at 13.2 μg/mL), Tribulus longipetalus (94.76% at 13.6 μg/mL), and Mentha longifolia, which had the lowest (81.94% at 18.4 μg/mL), whereas ascorbic acid, the standard, had the highest value (98.34% μg/mL). The antimicrobial properties of four selected MPs were evaluated. The plant T. longipetalus exhibited the highest zones of inhibition (18 and 20.5 mm) against Pseudomonas aeruginosa at concentrations of 12 and 24 mg/mL. The other plants also inhibited various fungi, including Alternaria alternata and Fusarium oxysporum, though to a lesser extent.

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