Isolation of Lactic Acid Bacteria from Local Medicinal Plants and Formulation of A Biologically Active Supplement Based on Them

In this study, over 50 lactic acid bacteria (LAB) isolates were obtained from Taraxacum officinale, Helianthus tuberosus, and Ajuga turkestanica. Taxonomic identification of 13 selected isolates was performed using MALDI-TOF mass spectrometry. Strains A. kunkeei 1, E. faecium 1, and L. plantarum (TK1, KA3, TK2) showed notable antioxidant activity, with free radical inhibition ranging from 55% to 93%. These strains produced amino acids linked to antioxidant defense - histidine, arginine, cysteine, and tryptophan - as well as vitamins B9, PP, and C. Carbohydrate monomers such as xylose and L-arabinose were also detected. L-arabinose, in particular, is known to reduce postprandial glycemic and insulinemic responses, indicating its metabolic significance. Moreover, exopolysaccharide (EPS) production by these strains may enhance their antioxidant properties and gut health benefits. Taken together, the studied LAB strains demonstrate probiotic potential with multifunctional properties, including antioxidant activity, vitamin biosynthesis, and metabolic versatility. These findings support their application in the development of biologically active supplements aimed at improving antioxidant defense, supporting gut microbiota, and potentially aiding glycemic control. Further in vivo studies are recommended to validate these effects.

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