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Enhancing growth and physiological traits in alfalfa by alleviating salt stress through biochar, hydrogel, and biofertilizer applications

Dilfuza JabborovaInstitute of Genetics and Plant Experimental Biology, Uzbekistan Academy of Sciences, Kibray, UzbekistanYu ZhangKey Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs/Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, ChinaSaleh S. AlhewairiniDepartment of Plant Protection, College of Agriculture and Food, Qassim University, Buraidah, Saudi ArabiaZаfаrjon JаbbarovFaculty of Biology, National University of Uzbekistan, Tashkent, UzbekistanJayanthi BarasarathiFaculty of Health and Life Sciences (FHLS), INTI International University, Nilai, MalaysiaTokhtasin AbdrakhmanovFaculty of Biology, National University of Uzbekistan, Tashkent, UzbekistanOtаmurod ImomovFaculty of Biology, National University of Uzbekistan, Tashkent, UzbekistanSokhibjon AbdusamatovFaculty of Biology, National University of Uzbekistan, Tashkent, UzbekistanBaljeet Singh SaharanDepartment of Microbiology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, IndiaR. Z. SayyedDepartment of Biological Sciences and Chemistry, College of Arts and Science, University of Nizwa, Nizwa, Oman
Frontiers in Microbiologyjournal2025en
ABI

Аннотация

Introduction: Salinity is a significant abiotic stress that affects the growth, physiology, and yield of crop plants across the globe. Sustainable measures to mitigate saline soil and prevent yield losses require immediate attention. The present study aimed to determine the impacts of hydrogel, biochar, and biofertilizer on alfalfa growth and physiological properties under salt stress. Methods: The experiment was performed in a randomized block design with three replications on the dried bottom of the Aral Sea, consisting of control (T1), hydrogel alone (T2), biochar alone (T3), and biofertilizer alone (T4). Plant growth parameters, root morphological traits, and physiological properties were analyzed after 60 days of sowing. Results: The results showed significant improvement in shoot length, shoot dry weight, and root dry weight in biochar alone (T3) and biofertilizer alone (T4) treated plants compared to control (T1) and hydrogel (T2). However, the application of biochar alone (T3) exhibited more pronounced effects compared to other treatments. Discussion: Biochar treatment resulted in the highest chlorophyll a and total chlorophyll contents under salt stress. Soil amendments with biochar, hydrogel, and biofertilizer promote alfalfa growth and yield and help mitigate the adverse impact of salt stress.

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