Study of quality and quantitative composition of Berberis iliensis and Berberis integerrima fruits extracts, preservation of the genetic material in cryobank

Two barberry species have been studied and preserved: Berberis iliensis M. Pop., Berberis integerrima Bunge. As a result of work the in vitro collection consisting of 33 accessions of these species was established. Obtaining in vitro collection was placed for medium-term storage at 4 ◦ C. Optimal duration of cold storage is 12 months. Cryobank of shoot tips and seeds was created. Cryobank of seeds created at temperatures: 1)-196 ◦ C, seeds germination after defrosting– 80 100%; 2)-20 ◦ C, seed germination after defrosting the average is 86,7%. Positive effect of storage at low temperatures on seeds germination was revealed. To study the qualitative and quantitative chemical composition 11 accessions of two barberry species were investigated. Fatty acids, fatty acids esters, alkanes, alkenes, alkadienes, aromatic hydrocarbons, aldehydes, fatty alcohols, triterpenoids, ketones, silicones, vitamins K, K 1 , K 2 were identified in studied accessions. In some barberry accessions carotenoid squalene, hydrocarbons 28-Nor-17-alpha and amide pyridine-3-carboximidamide were detected.

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